an overlay of all channels), each channel can be assigned a monochrome false-color LUT, e.g. To display a multi-channel fluorescence image in composite mode (i.e. by monochrome cameras or with photomultipliers): each channel contains just intensity values. More information on this topic can be found on the Color Image Processing page.įluorescence images are usually acquired without color information (i.e. When working with an image of higher bit depth, its intensity values are binned into 256 levels between minimum and maximum (see section Brightness/Contrast), and the LUT is applied onto these binned levels. Note: in the ImageJ 1.x user interface, LUTs are always 8-bit. The LUT Menu of ImageJ contains a large collection of lookup tables that can be applied to a pseudocolor image. Thus, differences in color in the pseudo-colored image reflect differences in intensity of the object rather than differences in color of the specimen that has been imaged. A LUT is a predefined table of gray values with matching red, green, and blue values so that shadows of gray are displayed as colorized pixels. Getting Started with Simple Visualization Options in ImageJ Pseudocolor Image Look-Up Tables (LUTs)Ī pseudocolor image is a single channel gray image (8, 16 or 32-bit) that has color assigned to it via a lookup table, i.e. 5 Interactive Analysis Plugins based on 'Big Data' Visualization Tools.4 Recommended ImageJ Plugins for 'Big Data' Visualization.3.1 Available Plugins for Making Figures in ImageJ.2.3 Plotting tools available via scripting.2.2 Available Plugins for Making Plots in ImageJ.1.3 Visualization of Volumetric Image Data.1.1 Pseudocolor Image Look-Up Tables (LUTs).1 Getting Started with Simple Visualization Options in ImageJ.
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